Antibiofilm Activity of Chromatographic Fractions from Extracts of Mangifera indica and Mentha piperita Leaves against the Clinical Isolates of Staphylococcus aureus
Authors
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Zubairu Maimuna
Department of Biochemistry, Faculty of Life Science Kaduna State University, Kaduna.
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Ahmed Aishat
Department of Biochemistry, Faculty of Life Science Kaduna State University, Kaduna.
Abstract
and therapeutic failure. This study investigated the inhibitory effects of chromatographically fractionated leaf extracts of Mangifera indica (mango) and Mentha piperita (mint) against biofilm formation by clinical isolates of staphylococcus aureus. Methanolic extracts of both plant materials were subjected to column chromatography to obtain distinct fractions, which were subsequently evaluated for antibiofilm activity using microwell diffusion assays in tryptic soy broth. Ciprofloxacin served as the positive control while DMSO was employed as the negative control. Among the mango extract fractions F3A demonstrated the most potent antibiofilm activity with 98.14 ≤ 0.53 % inhibition at 50 mg/ml. Similarly, the mint extract fraction F5 exhibited the highest biofilm inhibition at 86.94 ± 0.51 %. Notably, fraction 1 yielded the highest percentage recovery among all fractions tested. These results indicate that M. indica and M. piperita leaf extracts contain bioactive compounds with significant antibiofilm properties against S. aureus. The finding support the traditional antimicrobial applications of these plants and suggest their potential as sources of novel antibiofilm agents. Further studies to identify the specific bioactive compounds and elucidate their mechanisms of action are required.
Keywords: Antibiofilm: Chromatography: S. aureus: Resistance: Bioactive